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Figure 6 | Algorithms for Molecular Biology

Figure 6

From: AlignMiner: a Web-based tool for detection of divergent regions in multiple sequence alignments of conserved sequences

Figure 6

Use of AlignMiner for designing several specific primer pairs for PCR amplification of the different isoforms of the AtGS1 nucleotide sequence (A) The 5' and 3' divergent regions obtained with Entropy that were selected for primer design including the characteristic parameters of each region. (B) Results of the in silico "PCR amplification" with BioPHP [34] using the different primer pairs. Note that the actual 3' primers are complementary to the sequences shown on the right.

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Algorithms for Molecular Biology

ISSN: 1748-7188