Figure 8

Analysis of two Pinus pinaster gene isoforms. The specific primer pair for the photosynthetic isoform is identified by a "P" and for the non-photosynthetic isoform by an "N" (A) Predicted sequence and properties of the two primer pairs designed for specific identification of each isoform. (B) PCR analysis using the previously-predicted primers. Table 2 includes the expected amplicon size using these primer pairs. The template in the different lanes is: cDNA for the photosynthetic isoform (lanes 1), cDNA for the non-photosynthetic isoform (lanes 2), cDNA synthesised from total mRNA extracted from Pinus pinaster (lanes 3), Pinus pinaster genomic DNA (lanes 4), and negative controls (lines 5), which do not contain any DNA. Lanes M are molecular weight markers (vector pFL61 digested with Hpa II). Arrows indicate the specific amplification bands. DNA sizes are given in base pairs.